anti cd36 abd serotec  (Bio-Rad)

Journal: Endocrinology

Article Title: Perinatal Bisphenol A Exposure Increases Atherosclerosis in Adult Male PXR-Humanized Mice

doi: 10.1210/en.2017-03250

Figure Lengend Snippet: Antibody Table

Article Snippet: Species Raised in; Monoclonal or Polyclonal Dilution Used RRID Histone 3 lysine 4 trimethyl Proprietary Anti-H3K4me3 Abcam, ab8580 Rabbit; polyclonal 2.5 μg/5 μg DNA (ChIP) AB_306649 Histone 3 lysine 27 trimethyl Proprietary Anti-H3K27me3 Abcam, ab6002 Mouse; monoclonal 2.5 μg/5 μg DNA (ChIP) AB_305237 CD36 Proprietary Anti-mouse CD36 Bio-Rad/AbD Serotec, MCA2748 Rat; monoclonal 1:100 AB_2072640 Rat IgG Whole IgG Texas Red goat anti-rat IgG (H+L) Vector Laboratories, TI-9400 Goat antibody 1:500 AB_2336204 Open in a separate window Abbreviations: AB, antibody; H, heavy (chain); IgG, immunoglobulin G; L, light (chain); RRID, Research Resource Identifier.

Techniques: Sequencing, Plasmid Preparation

Journal: Endocrinology

Article Title: Perinatal Bisphenol A Exposure Increases Atherosclerosis in Adult Male PXR-Humanized Mice

doi: 10.1210/en.2017-03250

Figure Lengend Snippet: Perinatal BPA exposure increases aortic and atherosclerotic lesional CD36 expression in adult male huPXR•ApoE−/− mice. (A) Messenger RNA (mRNA) levels of PXR target genes were measured in aorta of male huPXR•ApoE−/− and PXR−/−ApoE−/− offspring by qPCR (n = 5 to 6; *P < 0.05). (B) Sections of atherosclerotic lesions in the aortic root of male huPXR•ApoE−/− and PXR−/−ApoE−/− offspring were stained with anti-CD36 primary antibodies, followed by fluorescein-labeled secondary antibodies. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar = 100 μm.

Article Snippet: Species Raised in; Monoclonal or Polyclonal Dilution Used RRID Histone 3 lysine 4 trimethyl Proprietary Anti-H3K4me3 Abcam, ab8580 Rabbit; polyclonal 2.5 μg/5 μg DNA (ChIP) AB_306649 Histone 3 lysine 27 trimethyl Proprietary Anti-H3K27me3 Abcam, ab6002 Mouse; monoclonal 2.5 μg/5 μg DNA (ChIP) AB_305237 CD36 Proprietary Anti-mouse CD36 Bio-Rad/AbD Serotec, MCA2748 Rat; monoclonal 1:100 AB_2072640 Rat IgG Whole IgG Texas Red goat anti-rat IgG (H+L) Vector Laboratories, TI-9400 Goat antibody 1:500 AB_2336204 Open in a separate window Abbreviations: AB, antibody; H, heavy (chain); IgG, immunoglobulin G; L, light (chain); RRID, Research Resource Identifier.

Techniques: Expressing, Staining, Labeling

Journal: Endocrinology

Article Title: Perinatal Bisphenol A Exposure Increases Atherosclerosis in Adult Male PXR-Humanized Mice

doi: 10.1210/en.2017-03250

Figure Lengend Snippet: Perinatal BPA exposure alters histone modification in CD36 promoter. (A) Scheme of ChIP-qPCR primers that cover the PXR element (PXRE) in CD36 promoter. ChIP-qPCR analysis of (B) H3K4me3 and (C) H3K27me3 enrichment of CD36 promoter in aorta of male huPXR•ApoE−/− and PXR−/−ApoE−/− offspring (n = 3; *P < 0.05 and **P < 0.01).

Article Snippet: Species Raised in; Monoclonal or Polyclonal Dilution Used RRID Histone 3 lysine 4 trimethyl Proprietary Anti-H3K4me3 Abcam, ab8580 Rabbit; polyclonal 2.5 μg/5 μg DNA (ChIP) AB_306649 Histone 3 lysine 27 trimethyl Proprietary Anti-H3K27me3 Abcam, ab6002 Mouse; monoclonal 2.5 μg/5 μg DNA (ChIP) AB_305237 CD36 Proprietary Anti-mouse CD36 Bio-Rad/AbD Serotec, MCA2748 Rat; monoclonal 1:100 AB_2072640 Rat IgG Whole IgG Texas Red goat anti-rat IgG (H+L) Vector Laboratories, TI-9400 Goat antibody 1:500 AB_2336204 Open in a separate window Abbreviations: AB, antibody; H, heavy (chain); IgG, immunoglobulin G; L, light (chain); RRID, Research Resource Identifier.

Techniques: Modification, ChIP-qPCR